Worm gene name:  ugt-62
Worm sequence name:  M88.1
Related human gene:  UDP glucuronosyltransferase 1 family, polypeptide A1
Associated human disease:  Crigler-Najjar Syndrome, type II ( Arias Syndrome)
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Left primer sequence:  gtactcgtgtggccgaagtt
Right primer sequence:  agaagcccatttcatcatcg
Size of PCR product:  551
Brief description:  The human ortholog of the C. elegans ugt-62 gene is associated with an autosomal recessive genetic disorder in which bilirubin cannot be conjugated into its water-soluble form (bilirubin glucuronide). Homozygous recessive individuals fail to synthesize the active enzyme bilirubin glucuronyltransferase, resulting in hyperbilirubinemia and jaundice.
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Primers successfully amplified product of ~500nt. This product was successfully ligated into L4440 and transformed into DH5alpha.
Posted by Kristen Johnson on 2008-08-22 13:25:06