Worm gene name:  daf-2
Worm sequence name:  Y55D5A.5
Related human gene:  Insulin receptor
Associated human disease:  Type II Diabetes
People involved in this project: 
Left primer sequence:  gtttcggaccgtgtgctatt
Right primer sequence:  agaacaactccgaagctcca
Size of PCR product:  541
Brief description:  C. elegans with defective daf-2 correspond to insulin resistance and Type II diabetes.
Report any problems that might have appeared and any solutions: 

I tried to use the primers to make an amplicon at 50 and 55 degree C annealing temp. The PCR did not work at all and I had no band visible in the gel.
Posted by Sanghamitra Mohanty on 2008-08-22 13:31:29