Worm gene name:  spe-15
Worm sequence name:  F47G6.4
Related human gene:  MYO6
Associated human disease:  deafness
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Left primer sequence:  gttttggatgtcgctggttt
Right primer sequence:  tctcgtctcatagcacaccg
Size of PCR product:  414
Brief description:  The spe-15 gene encodes an unconventional myosin that is homologous to both Drosophila and mouse myosin VI; it also has homologues in many other organisms. spe-15 was identified in C. elegans in screens for defective spermatogenesis. The encoded protein is involved in organelle transport and endocytic trafficking. A missense allele of the human ortholog MYO6 causes a dominant form of sensorineural deafness while other alleles are recessive.
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Successfully amplified target sequence with gene-specific primers. However, was not able to successfully ligate into L4440 or pGEMT vectors.
Posted by Janice Lai on 2008-08-22 13:18:20