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Worm
and Bacterial Strains
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Dear Silencing Genomes users, After over ten years supporting strain orders through Silencing Genomes, we regret to announce that we will no longer be providing strains of E. coli or C. elegans to support RNAi experiments. A wide range of strains for use in these experiments are available from strain banks, so we hope that everyone will be able to continue their teaching. For C. elegans strains, you can order directly from the Caenorhabditis Genetics Center (CGC). The CGC has a very large collection of worm strains and set of standard bacterial strains for worm culture available for a subsidized price through support from the National Institutes of Health – details can be found on their website: https://cbs.umn.edu/cgc/strains. To order strains from the CGC, you need to follow the CGC’s instructions, which are found at the following site: https://cbs.umn.edu/cgc/how-order-strains C. elegans strains are named. To ensure you are ordering the correct strain, you need to order them using the strain name, not the gene name, as many genes have multiple mutations found in different strains. Also, many mutations are found in multiple strains. Below is a list of the common strains ordered from Silencing Genomes and the genotype (set of mutations or transgenes) found in each strain.
For RNAi plasmids, Addgene (https://www.addgene.org/1654/) has plasmids, including empty vectors and plasmids targeting many genes. For RNAi bacterial strains, two RNAi libraries exist: the Ahringer lab library and the Ambrose RNAi library. Individual strains or collections of thousands of clones can be obtained from Source Bioscience: Once you have obtained strains, to avoid future cost you can freeze both C. elegans and bacterial strains and store at -80°C. For most, we hope that you have access to a -80°C or a local laboratory can help with storage. For worm strains, you can follow the instructions in wormbook: For bacterial strains, addgene has a good protocol: Also, for some experiments on Silencing Genomes, kits including the strains are available through our partner, Carolina Biological:: Please contact us with any questions or concerns – we will do what we can to help. |
